The protein kinases controlling flagellar function in these mice are overexpressed and cause abnormal sperm motility however, those spermatozoa carrying the t-haplotype also possess a t-complex responder gene (t cr) that corrects the overexpression of the sperm motility kinase gene (smok) and restores normal flagellar action 81. zonadhesin is the inner-acrosomal protein in porcine sperm which is involved in sperm-oolemma interaction 31. Mice sperm-oolemma penetration was inhibited by anti-equatorin (mn9-antigen, a transmembrane sialoglycoprotein) antibody without affecting sperm motility or zona binding, which proves its role in sperm-oocyte penetration 36. Protein phosphorylation has been associated with sperm capacitation and the acrosome reaction in several species (duncan & fraser 1993, visconti et al. 1998), so it is an interesting question whether 5-ht could promote protein phosphorylation in human sperm. Sperm motility is also affect ed by immunological factors high levels of cyt okines such as tumor necrosis factor alpha, interleukin 6 and interleukin 1 beta in seminal. In addition, the present study explored the correlation between sperm lysine 2-hydroxyisobutyrylation and sperm motility, as well as the role that lysine 2-hydroxyisobutyrylation plays in asthenozoospermia, which is a common disorder consisting of sperm motility defects that account for almost half of male infertility cases (saraswat et al.). It is true that proteins in male sperm help in its motility, and tie the sperm to the female egg. This is why the right measures must be put in place to prevent premature ejaculation and enhance the protein amounts in the sperm. Barrenness of specific sorts emerges from the corruption of protein in sperm. Defective sperm function is known as one of the common causes of human infertility. There are many studies suggesting a relationship between reactive oxygen species and sperm function impairment (1 x 1 aitken, r. Superoxide dismutase in human sperm suspensions (relationship with cellular composition, oxidative stress, and sperm function). Protein-rich foods, particularly red meat, have gained a reputation for being masculine foods that add to male potency and vitality. thereafter, sperm concentration and motility were evaluated using makler chamber and light microscope (olympus ckx 41 germany) equipped with a 10 objective (cachn na 0. 10 l of sperm suspension was pipette to the makler chamber, thereafter spermatozoa were counted in 3 lines, each of 10 squares and divide by 3 to obtain average sperm.). Effect of recombinant defensin 1 protein on human sperm motility and viability. Level may particularly affect the post-testicular events of sperm maturation that lead to fully infertile.